AUTOPHAGOSOME CONTENT PROFILING USING PROXIMITY BIOTINYLATION PROTEOMICS COUPLED TO PROTEASE DIGESTION IN MAMMALIAN CELLS

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells

Summary: The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest Briefs by proximity-dependent biotinylation.Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes and potentially other short-lived

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Effects of defects on laminate quality and mechanical performance in thermoplastic Automated Fiber Placement-based process chains

Automated Fiber Placement of thermoplastic unidirectional tape materials offers several advantages over conventional organosheets, such as enhanced part performance through tailored fiber architecture, and economic and ecological benefits due to scrap reduction.Because Discontinued material is cut perpendicular to the feeding direction in state-of-

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The Outstanding Chemodiversity of Marine-Derived Talaromyces

Fungi in Silicone Spray the genus Talaromyces occur in every environment in both terrestrial and marine contexts, where they have been quite frequently found in association with plants and animals.The relationships of symbiotic fungi with their hosts are often mediated by bioactive secondary metabolites, and Talaromyces species represent a prolific

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